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To further explore the molecular mechanism of AA-CDs inhibiting chondrocyte ferroptosis, we employed the Drug Affinity Responsive Target Stability (DARTS) assay to identify potential direct target proteins of AA-CDs [49, 50]

The glutamic-acid substitution sterically prevents binding to IGF-binding proteins (IGFBPs), leaving a far larger fraction of the peptide free to engage the IGF-1 receptor (IGF-1R)

Most people can safely take up to 200 milligrams daily (usually split into two to three doses), although higher doses around 400 milligrams have also been used safely

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