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Specific primers used for PCR amplification were synthesized with the sequences as shown in Table 1 The relative expression levels of target genes were analyzed using the 2 Ct method

Specifically we found that the smiliside A/smigliside C ratio can only be increased by 1-aminocyclopropanecarboxylic (1-ACC, an ethylene biosynthetic precursor) in near isogenic lines that carry the Mo17 allele at this locus

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Ferrostatins inhibit oxidative lipid damage and cell death in diverse disease models