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Description

The coupling agent in step a), step c) and step e) is selected from those described above

Competent yeast cells were transformed with yeast-display plasmid pCTCON2 following the Frozen EZ yeast transformation II (Zymo Research, T2001) according to the manufacturers protocol

Additionally, a basal metabolic rate is calculated based on your direct measurements, not through statistical analysis which most composition scales use