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Simultaneous measurement of folate cycle intermediates in different biological matrices using liquid chromatographytandem mass spectrometry

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The citric acid cycle involves three NAD+-dependent dehydrogenase reactions: isocitrate dehydrogenase, -ketoglutarate dehydrogenase, and malate dehydrogenase, each generating NADH while releasing carbon dioxide and regenerating oxaloacetate for continued cycle operation

It remains uncertain whether dysregulated choline distribution affects disease progression, and significant gaps exist in understanding how cells adapt to impaired choline availability or metabolism locally