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Cells were counted and resuspended in proliferation media at a concentration of 100,000 cells per well for 24-well transwells (Corning), and 150,000 cells per 48-well plates

But eligibility under IRS rules is the floor, not the ceiling

Interventions At first, the principal investigator (MK) explained the potential benefits of the current trial and obtained informed consent from their patients or their legal representatives

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