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DNA fragments were purified using Qiaquick miniElute (Qiagen) according to the manufacturer protocol and diluted to 1/10 for input and 1/2 for immunoprecipitated fractions

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Predefined sensitivity analyses to determine potential confounding influence were conducted with the same statistical analysis set-up removing (1) patients with IIH without papilledema (IIH-WOP), and (2) patients with pre-existing migraine

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