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Approximately, half of the pelleted cells were lysed by incubation with 120 l CHAPS (Merck Millipore, including 0.15 units/l RiboLock [LifeTechnologies, Thermo Fisher Scientific]) on wet ice for 30 min and three short vortexes, thereafter the lysate was stored at 80 C

Researchers associate a vitamin B12 deficiency with various health issues, megaloblastic anemia neurological changes and disorders infertility depression neural tube defects and developmental delays in children born to those with a vitamin B12 deficiency Supplementation may help to reduce the risk of these health issues

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