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Second, although numerous observational studies have shown causal associations between TMAO and cardiovascular, kidney, neurological, and metabolic diseases, due to the inherent limitations of statistical methods (bias due to confounding factors and reverse causality), and the causal relationship between TMAO and human diseases still requires validation through multicenter, multi-ethnic randomized controlled trials

Hence, we reassessed the expression of the aforementioned markers in tissues with and without synapses

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