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Unaffected individuals from both cohorts obtained from Kahemba were not considered to have konzo at the time of sampling following the 3 main criteria for diagnosis outlined by the World Health Organization

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Immunofluorescence staining and western blot results verified that GPX1 expression was absent in the cochlear SGNs of gpx1 / mice (Supplementary Figures 1A,C), and there was no significant difference in SGNs number between the gpx1 / mice and WT mice (Supplementary Figure 1B), which indicated that the absence of GPX1 in normal SGNs would not affect the survival of SGNs