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These results collectively exclude protein degradation as a cause for AMPK protein reduction in shGDH1 cells

These minimum criteria are an example of what veterinary researchers call "quantitative measurement methods" , which are designed to dumb-down the level of professionalism which one otherwise would expect from both investigative and clinical veterinarians in performing their examinations

For NAC intervention experiments, rats were co-treated with N-acetylcysteine (NAC, Sigma Aldrich

The cell culture environment was kept at 37 with 5% carbon dioxide and humidity, and the culture medium (DMEM) was refreshed every 23 days