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In these conditions, the iron-binding capacity of serum transferrin is exceeded, resulting in low affinity complexes of iron with other plasma components (Porto and De Sousa, 2007

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Briefly, urine samples (500 l), HepaRG cell and hepatocyte samples (300 l), and microbiota samples (150 l) were diluted to 1 ml of HPLC grade water, acidified with 1% formic acid and extracted using Strata-X SPE

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