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Kernohan AF, McIntyre M, Hughes DM, Tam SW, Worcel M, Reid JL

The native or two modified GLP-1 (mGLP-1) with Ala8Gly substitution or Ala8Gly, Lys26Gln, and Lys34Asp substitutions were genetically fused to the N-terminus of HSA-binding DARPin through a rigid helical linker (EAAAK) 3 to create the fusion proteins, denoted as nGLP1-DARPin, mGLP1-DARPin-1, and mGLP1-DARPin-2, respectively

Then the blots were detected with HRP conjugated secondary antibody and visualized with the Super ECL Detection Reagent ECL (#36208ES60, Yeasen)