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Generation and quantification of GSH, GSSH, persulfide and polysulfides from reaction of GSH and Na 2 S GSH (10 M) in 400 L of 50 mM HEPES buffer (pH 7.4) was reacted with Na 2 S 2 (1030 M) for 5 min at RT in a 2 mL capacity 96-well plate under argon sealed with parafilm

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Others drink occasionally in moderation throughout treatment without problems