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In this paradigm, aquaporin 8 silencing, which is specifically expressed in IMM, enhances mitochondrial ROS generation and results in mitochondrial depolarization and cell death ( 2 O 2 at higher rates than complex I from mitochondria (Quinlan et al., 2014)

doi: 10.1001/jama.260.7.971 144 StrausSEDaleJKTobiMLawleyTPrebleOBlaeseRMet al

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Using the BODIPY C11 assay, we observed that hypoxia increased lipid peroxidation in cultured proliferating and differentiating chondrocytes of both genotypes, but the most pronounced increase was observed in G6PDH-null differentiating hypoxic chondrocytes, which display the highest ROS levels (Extended Data Figs